Abstract
As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips’ image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree (R2) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing.
Highlights
Immunochromatographic assay (ICA) [1] combines the chromatographic assay and the specific reaction of antigen and antibody
The effect of automatic range adjustment of the fluorescence immunochromatographic assay (FICA) reader based on image processing is shown
Automatic range adjustment of the FICA reader based on image processing is shown The effect in Figures 7 andof
Summary
Immunochromatographic assay (ICA) [1] combines the chromatographic assay and the specific reaction of antigen and antibody. With biomarkers as the tracer, useful information can be obtained directly or indirectly through optical instruments to achieve quantitative detection of the analyte. There are mainly three kinds of biomarkers in the ICA applications, including nano-gold [9], quantum-dot [10,11], and fluorescent particles [12,13]. Sensors 2020, 20, 209 nano-gold, fluorescence immunochromatographic assay (FICA) can be used for quantitative determination of the analyte. The existing FICA research can be classified into photoelectric and image detection. Photoelectric FICA is used to detect melamine in milk [14]; the range of the reader was previously adjusted by controlling the light intensity [15]. This paper designed a portable FICA system based on auto fluorescence imaging. Fluorescence imaging technology has been applied in clinical trials [17]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call