Abstract
We propose an automatic high-order aberration correction method for digital holographic microscopy based on orthonormal polynomial fitting over an irregular-shaped aperture. The corner detection technique is used to detect the specimen-free area for orthonormal polynomials over an irregular-shaped aperture. The high-order aberration correction method is completely automatic and requires only a single hologram. Experiments showed that in aberration correction, both lower-order phase curvatures and high-order aberrations could be corrected without requiring extra devices. Numerical simulations show that the proposed method provides more accurate high-order aberration correction than aberration correction methods based on least squares fitting with standard polynomials. Experimental results demonstrate the feasibility of using the proposed method for analyzing human macrophage cells.
Published Version
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