Abstract
Measurement of ethanol in blood, urine and saliva are of great practical importance for clinical purposes [l]. Hence, numerous analytical methods have been developed to achieve fast, accurate and selective determinations of this substance. Such methods may be classified as chemical [2], gas chromatographic [3,4] and enzymatic, using immobilized [5-71 or dissolved enzymes with [8] or without another coupled enzymatic reaction [9,10]. Enzymatic methods have clear advantages over those mentioned above, such as high specifity and the need for no separation processes. Photometry is the most frequently used detection technique for these methods, followed by electroanalytical techniques, whilst fluorimetry has been used in only two papers [5,11]. We have chosen this last type of detection to develop determinative methods for the determination of ethanol in blood based on the reaction:
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