Abstract
Phase aberrations must be precisely compensated for phase-contrast imaging in digital holographic microscopy. In this paper, an automatic and accurate phase aberration compensation method is proposed based on iteratively reweighted least squares fitting. Instead of selecting the known-to-be-flat region with manual operation or image segmentation, this method starts from conventional least squares fitting in the whole image and attributes lower weights to the pixels with higher fitting residuals in an iterative reweighting procedure, during which the influence of specimen and noise is automatically reduced. Both phase curvature and high-order aberrations could be corrected without extra operation. Numerical simulation demonstrates that the proposed method is more accurate than the state-of-the-art numerical methods. Experimental result proves that this method is robust and fast enough for dynamic imaging of living cells.
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