Abstract
The repetitive analysis of serum lactate dehydrogenase (LDH) isoenzymes has been performed on a weak anion exchanger (TSKgel DEAE-5PW), which was developed by introducing diethylaminoethyl groups into TSKgel G5000PW (10 μm particle diameter) — a hydrophilic polymer-based material of large pore size — for high-performance gel chromatography. By use of this anion exchanger, a high-pH (8.0) solvent could be used and the albumin peak was completely separate from the LDH isoenzyme peaks. After 10 successive analyses with an autosampler, the coefficient of variation of the LDH isoenzyme elution times was ⩽ 0.90%, and the coefficient of variation for peak areas was ⩽ 3.85%. After 40 successive analyses, resolution between isoenzymes was generally 1.25. This column can be used for more than 300 intermittent injections of human serum.
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