Abstract
Abstract The direct procedure for determining serum cholesterol described by Wybenga et al. [Clin. Chem. 16, 980 (1970)] can be used to assay 29 samples in 10-min reaction time by using the "CentrifiChem" parallel fast analyzer (Union Carbide). Commercial reference serum was used for standardization. Correlation of results with those obtained by the method of Abell et al. [J. Biol. Chem. 195, 357 (1952)] for 54 samples yielded a regression line with a slope (m) of 0.897 and a y-intercept (b) of 231 mg/liter; the correlation coefficient (r) was 0.971. Compared with the manual procedure of Parekh and Jung [Anal. Chem. 42, 1423 (1970)] for 146 samples, the corresponding values were m = 0.991, b = 27 mg/ liter, and r = 0.981. Compared to serum extracts assayed with the "AutoAnalyzer" (Standard Technicon Methodology N-24a), 186 samples gave corresponding values of m = 0.990, b = 92 mg/ liter, and r = 0.979. No bias significant at the 95% confidence level existed versus any of the three methods. Sera containing abnormally high bilirubin concentrations (>50 mg/liter) or abnormally high triglyceride content (>5,000 mg/liter) did not give significantly different cholesterol values on the CentrifiChem as compared with values obtained with either the AutoAnalyzer or the Parekh and Jung method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
