Abstract
Nucleic acid (NA) detection is essential across biological research, clinical diagnostics, and industrial applications, yet the complexity of traditional extraction, amplification, and detection processes limits their efficiency and scalability. This study presents an integrated, and fully automated microfluidic system for NA detection, designed to operate a seamless 'sample-in, result-out' workflow. By incorporating two rotary shafts and pre-loaded reagents on-chip, this system reduces human intervention, minimizes contamination risks, and accelerates the process of multiplex pathogen detection. Equipped with 12 reaction chambers and a four-color fluorescence detection module, the system enables simultaneous detection of up to 48 unique targets under 80 min, achieving detection sensitivity as low as 10 CFU/mL in spiked samples. This system’s efficiency and robustness indicate promising applications in medicine, public health, agriculture, forensic science, and other research domains.
Published Version
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