Abstract
Summary: When using a spectrophotometric method to measure cholinesterase activity in carbamate-treated tissues, precautions must be taken to limit the reactivation of the inhibited cholinesterase. Many testing laboratories use automated instruments to measure cholinesterase activity, instruments that usually employ a spectrophotometric method. To date, there has been no systematic investigation of the validity of the cholinesterase data obtained from carbamate-treated tissues using these automated instruments. The purpose of this study was to compare the cholinesterase data obtained using an automated analyzer with those obtained using a radiometric assay (i.e., the optimal method for unstable inhibitors). Using both an automated analyzer and the radiometric method, cholinesterase activity was measured in whole blood and brain tissue taken from rats treated with various dosages of carbaryl. Reactivation occurred when using the automated instrument. In the brain tissue, cholinesterase activity of the trea...
Published Version
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