Abstract
A pressure-based variable-bed flow reactor built for peptide synthesis and capable of real-time monitoring of resin swelling was adapted for automated glycan assembly. In the context of the solid-phase synthesis of several oligosaccharides, the coupling efficiencies, resin growth patterns, and saccharide solvation during the synthesis were determined. The presented work provides the first estimation of on-resin oligosaccharide solvation and an alternative technique to UV–vis monitoring.
Highlights
A pressure-based variable-bed flow reactor built for peptide synthesis and capable of real-time monitoring of resin swelling was adapted for automated glycan assembly
Automated solid-phase synthesis is a facile way to assemble biopolymers, such as oligonucleotides, peptides, and oligosaccharides.[1−3] Insights into the interplay of peptide, resin, and solvent have proven fundamental to advancing automated solid-phase peptide synthesis (SPPS)
Automated continuous-flow peptide synthesis has benefitted from a variable-bed flow reactor (VBFR) system that allows for the synthesis cycle to be monitored in real time.[7−10] For that purpose, differential pressure changes across the reaction bed caused by resin swelling and shrinking are measured
Summary
Sletten − Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany. José Danglad-Flores − Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
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