Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations

Idrees F Al-Momani

doi:10.11648/j.ijpc.20170304.11

Abstract

A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.

Highlights

Omeprazole, 5-methoxy-2-[[(4-methoxy-3,5-dimethylpyridinyl) methyl] sulfinyl] -1 H- benzimidazole, is a gastric acid pump inhibitor, dose dependently controls gastric acid secretion by altering the activity of H+/ K+ ATPase, which is the final common step of acid secretion in parietal cells [1, 2]
Several methods such as high-performance liquid chromatography (HPLC) [3, 4], liquid chromatography–mass spectrometry [5], voltammetry [6] and spectrophotometry [7,8,9,10] have been developed for the determination of omeprazole in pharmaceutical product and biological fluids
The Flow Injection Analysis (FIA) technique has found recently wide applications mainly due to reduction of the analysis time and reagents consumption compared with conventional manual procedures

Summary

Introduction

Omeprazole, 5-methoxy-2-[[(4-methoxy-3,5-dimethylpyridinyl) methyl] sulfinyl] -1 H- benzimidazole, is a gastric acid pump inhibitor, dose dependently controls gastric acid secretion by altering the activity of H+/ K+ ATPase, which is the final common step of acid secretion in parietal cells [1, 2]. It is used for the treatment of acid-peptic diseases such as duodenal, gastric and esophegeal ulceration [2]. This paper describes a FIA method for the determination of omeprazole in pharmaceutical products and human plasma

Chemicals

Instrumentation and Analytical Conditions

Development of the Experimental Conditions

Evaluation of the Proposed Method

Analysis of Real Samples

Conclusions