Abstract
Abamectin is a drug with antiparasitic properties used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for quantification of the two abamectin homologs (H2B1a and H2B1b) in gel formulation. This HPLC method was validated using a LichroCart(r) 100 RP-18 (125 x 4 mm, 5 µm) column. The mobile phase contained of acetonitrile and water (95:5 v/v) with 1% acetic acid. The flow rate was 1.0 mL min-1 and UV detection was performed at 245 nm. Mobile phase solutions were prepared containing a nominal concentration 185.2 µg mL-1 H2B1a and 9.6 µg mL-1 H2B1b. The method displayed good linearity in the concentration range of 148.1 - 222.3 µg mL-1 and 7.7 - 11.5 µg mL-1, for H2B1a and H2B1b, respectively, with a correlation coefficient of (r)>; 0.99 for both compounds, calculated by the least mean squares method. Detection limits (DLs) were 2.8 µg mL-1 and 1.2 µg mL-1 and quantitation limits (QLs) were 8.6 µg mL-1 and 3.8 µg mL-1, for H2B1a and H2B1b, respectively. The method is simple, economical and efficient for the quantitative determination of abamectin H2B1a and H2B1b homologs in pharmaceutical preparations.
Highlights
Avermectins are a group of fermentation products obtained from a strain of Streptomyces avermitilis and are 16-membered macrocyclic lactones, and comprise several derivatives including abamectin (Xie et al, 2011)
Metabolic studies, have shown that abamectin H2B1b is generally metabolized more rapidly than the H2B1a homolog and H2B1a is the major residue found in tissues and biological fluids (Markus, Sherma, 1992)
The aim of this research was to validate a high performance liquid chromatographic (HPLC) method for quantitative determination of abamectin homologs (H2B1a and H2B1b) in gel formulation, as this drug is widely used in veterinary practice
Summary
Avermectins are a group of fermentation products obtained from a strain of Streptomyces avermitilis and are 16-membered macrocyclic lactones, and comprise several derivatives including abamectin (Xie et al, 2011). Abamectin (Figure 1) is used in veterinary practice as an antiparasitic and in agriculture as an insecticide. Abamectin consists of a mixture of two homologous compounds: H2B1a (at least 80%) and avermectin H2B1b (no more than 20%) (Shoop, Mrozik, Fisher, 1995). Metabolic studies, have shown that abamectin H2B1b is generally metabolized more rapidly than the H2B1a homolog and H2B1a is the major residue found in tissues and biological fluids (Markus, Sherma, 1992). Abamectin was suspected causing adverse effects in the.
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