Abstract
We have developed a method of analyzing fluorescence signals from optically thick tissue, which can be used to extract important clinical information about tissue type and chemical composition. Our model describes the composite tissue spectrum in terms of contributions from fluorophores and absorbers which modulate the intrinsic fluorescence spectrum. Scattering is included empirically in the model by describing absorbing species with ‘attenuation spectra’, which include contributions from both absorption and scattering. We present an analysis of laser induced fluorescence data obtained from normal and atherosclerotic human artery wall in vitro. We show that the model can be used to separate effects due to fluorescence and absorption, and to deconvolute contributions from individual chromophores, extracting information about their relative concentrations. This information can be utilized to diagnose tissue type more accurately than empirical algorithms, and may potentially prove to be useful in determining the chemical composition of atherosclerotic lesions in vivo.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.