Abstract
An increasing application of DNA fingerprinting in modern food biotechnology is the authentication of species and cultivars in commercial edible products. In this work we describe the genetic authentication of apple fruits from “Annurca” and “Annurca Rossa del Sud”, the leading varieties of the Italian Campania region, in two different highly-processed foodstuffs, nectar and purée. The identification was based on fluorescent-based capillary electrophoresis and automated size estimation of polymorphic DNA microsatellites. We selected 4 Simple Sequence Repeats (SSRs) able to distinguish 17 different apple accessions. Each SSR proved to be suitable to authenticate the presence of Annurca as ingredient in the analysed apple derivatives. This study represents the first application of DNA technology to the identification of apple cultivars in the agro-food chain.
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