Abstract
Here we reported the gold nanoparticles (GNPs) decorated ZnO thin films based biosensor for the detection of pork DNA hybridization. Label-free DNA detection is increasingly important in bio diagnostics, food analysis and forensic investigations. The capacitance, permittivity and impedance profiles of the biosensor clearly differentiated the complementary and non-complementary target DNA detection using low-cost dielectric analyzer. In this study, GNPs were used to create an attachment surface between the ZnO thin films and the thiol-modified probe DNA and to enhance the sensitivity of the biosensor up to ~59 %. The ZnO thin films and 35 ± 5 nm in diameters of GNP-coating on their surfaces were confirmed with various types of measurement such as FESEM, XRD, EDX and UV---Vis spectroscopy.
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