Abstract
Bovine tuberculosis is a chronic inflammatory disease that causes granuloma formation. Characterization of granulomatous lesions of Mycobacterium bovis (M. bovis) experimentally infected cattle has helped to better understand the pathogenesis of this disease. However, few studies have described granulomas found in M. bovis naturally infected cattle. The aim of this work was to examine granulomas from Holstein-Friesian cattle naturally infected with M. bovis from a dairy basin located in the central region of Mexico. Tissue samples from thirty-two cattle with lesions suggestive of tuberculosis were collected post-mortem. Fifteen of the 32 sampled animals (46.8%) were 4 months of age or younger (calves), whereas the rest (53.2%, 17/32) were over one year old (adults). Macroscopic lesions suggestive of tuberculosis were found in the mediastinal lymph node chain of all animals (32/32). From the 1,143 granulomatous lesions that were microscopically analyzed, 34.6% (396/1143) were collected from adult animals and subsequently classified according to the nomenclature suggested by Wangoo et al., 2005. Surprisingly, lesions from calf tissues showed an atypical pattern which could not be fitted into the established developmental stages of this classification. Granulomatous lesions found in calves covered most of the affected organ, histologically showed large necrotic areas with central calcification, absence of a connective tissue capsule, and few giant cells. Also, there was a higher percentage of lesions with acid-fast bacilli (AFB) when compared to studied granulomas in adults. Growth of Mycobacterium spp was detected in 11 bacteriological tissue cultures. Genotypic identification of M. bovis was performed by DNA extraction from bacterial isolates, formalin-fixed and paraffin-embedded (FFPE) tissues and samples without bacterial isolation. M. bovis was detected by PCR in 84.3% (27/32) of the studied cases; whereas other AFB were observed in tissues of the remaining sampled animals (5/32). Our results describe atypical granuloma formation in calves 4 months of age or younger, naturally infected with M. bovis. These findings contribute to better understanding the physiopathology of M. bovis infection in cattle.
Highlights
Bovine tuberculosis is a disease caused by M. bovis
It has been estimated that there are over 50 million infected cattle worldwide, generating losses of close to 3 billion dollars per year [1,2,3,4]. bTB is characterized by a chronic inflammatory process, which mainly affects the lungs and lymph nodes associated with the respiratory system, characterized by formation of caseous and necrotizing granulomas
Granulomas have been classified in M. bovis experimentally infected cattle according to morphological criteria such as degree of necrosis and mineralization, and presence of a connective tissue capsule [8]
Summary
Bovine tuberculosis (bTB) is a disease caused by M. bovis. It affects a wide range of mammals including humans, creating public health problems as well as economic losses for the livestock industry, due to factors such as low milk production, organ condemnation during post-mortem examination, and costs of eradication programs. Granulomas are considered the pathognomonic lesions of tuberculosis Their formation represents an attempt by the host to isolate and contain mycobacteria, as well as to limit further surrounding tissue damage by dampening chronic inflammation [6]. Lesions and tissues surrounding granulomas have been examined [9,10,11]. The aim of this study was to examine granulomatous lesions of M. bovis naturally infected Holstein-Friesian cattle. All granulomas found in tissues from 15 calves 4 months of age or younger, showed atypical structures
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