Abstract
Abstract The current study was conducted to evaluate the different concentrations of deltamethrin (DEL; Exp. 1) and the protective role of idebenone (IDB) supplemented with toxic dose of deltamethrin (Exp. 2) during chilled storage of ram semen. Collected samples were pooled and diluted at 500×106 spermatozoa per mL. In Exp. 1, effect of DEL at 1, 2.5, 5, 10, 20, 40, 80 μM levels was evaluated on different variables of spermatozoa motion characteristics. In Exp. 2, different amounts of IDB (2, 4, and 8 μM) concurrent with constant doses of DEL (10 μM) were examined on semen quality upon chilled preservation up to 72 h. Indices of spermatozoa kinematics, functionality of plasma membrane and viability were recorded. Biochemical metabolites were measured in spermatozoa and its medium (extender) at different time points. In Exp. 1, different parameters of spermatozoa kinematics were affected by exposure to DEL in a dose dependent manner. In Exp. 2, combination of IDB with DEL resulted in a significant (P<0.05) increase in total motility, forward progressive motility and curvilinear path velocity compared to DEL group at 24, 48 and 72 h. Simultaneous administration of IDB with DEL increased the percentage of spermatozoa with functional membrane and viability compared to DEL group at 72 h (P<0.05). The amounts of lipid peroxidation index were lower in medium of combination groups compared to DEL group at 48 h and 72 h (P<0.05). Antioxidant capacity was higher in spermatozoa and medium of IDB treated groups compared to DEL group at 72 h (P<0.05). Amounts of total nitrate-nitrite and superoxide dismutase activity of spermatozoa and medium were not affected by treatment (P>0.05). In conclusion, IDB could ameliorate oxidative and peroxidative damages induced by DEL mild toxicity upon cold preservation of ram semen.
Highlights
Ram spermatozoa is very susceptible to produced redox-active oxygen species during cooling, freezing and thawing process
The present study indicated that: (1) DEL reduced the total motility (TM) and forward progressive motility (FPM) of chilled stored ram spermatozoa in a dose dependent manner; (2) idebenone attenuated the oxidative toxicity of stored ram semen caused by DEL
Our results revealed that DEL reduced spermatozoa total motility and progressive motility in a dose dependent manner, and the findings are in accordance with the previous study (Park et al, 2017)
Summary
Ram spermatozoa is very susceptible to produced redox-active oxygen species during cooling, freezing and thawing process. It was documented that IDB reacts with different reactive oxygen and nitrogen species and reduced the harmful effects of them (Cardoso et al, 1998; Mordente et al, 1998; Erb et al, 2012). Protective role of IDB against oxidative stress during liquid-chilled and cryopreserved storage of ram spermatozoa was reported (Eslami et al, 2019; Lone et al, 2019). Protective role of quercetin against induced toxicity by deltamethrin on reproductive system of male rats was reported (Sharma et al, 2018). The first aim of the present study was to investigate the effect of different levels of DEL on ram semen kinematics during storage at 4°C. After choosing the mild toxic dose of DEL, the second objective was to assess the probable protective role of IDB against DEL-induced toxicity on different parameters of chilled stored semen
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