Abstract
Glucosinolates are the main bioactive compounds present in Brassicaceae family with remarkable protective activities against various chemical carcinogenesis and toxicities. Protective role of the hydroalcoholic extract and desulfo glucosinolate fraction from seeds of Lepidium perfoliatum were evaluated against CCl4-induced hepatotoxicity in BALB/c mice by determination of oxidative stress biomarkers and liver histopathological feature. The oral administration of different doses of the extract and desulfo glucosinolate fraction exhibited significant decrease in ROS and MDA contents of mitochondria in a dose-dependent manner, especially the doses of 200 mg/kg of the extract and 10 mg/kg of the fraction. GSH content was significantly increased (p<0.05) only in the group fed with 10 mg/kg of the fraction. Treatment groups showed notable reduction in ALT, AST and ALP levels. Based on the histopathological findings, the destructive effects of CCl4 were alleviated by the treated groups however the fraction (10 mg/kg) showed the best results. Based on NMR and EI-MS spectra, an alkyl glucosinolate bearing a thiofunction named glucoerucin was identified as the major constituent (80%) of the fraction. In addition, the GC-MS analysis revealed the presence of glucoerucin hydrolysis products, erucin and erucin nitrile, which representing 97.6% of the total seed volatiles. Based on the present study, it seems that glucoerucin is involved in the protection of L. perfoliatum against CCl4-induced hepatic damage via the inhibition of oxidative damage pathways.
Published Version
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