Abstract
Specific step-by-step instructions are given for coupling nucleosides to LCAA-CPG supports (supports consisting of a long-chain alkylamine linked to controlled-pore glass). Protocols are given for a succinic acid linker and a hydroquinone-O,O'-diacetic acid linker. The former is the most widely used linker arm, and the starting materials are widely available. The latter offers greater compatibility with base-sensitive sequence modifications and great synthetic throughput because it can be cleaved under milder and faster conditions. Additional guidelines are given for selecting a linker arm and coupling protocol. Almost any application requiring synthetic oligonucletodies can be satisfied using one of these linker arms.
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