Abstract
The present study evaluated the ability of Attenuated Total Reflectance – Mid-Infrared (ATR-MIR) spectroscopy combined with Partial Least Squares Discriminant Analysis (PLS-DA) to discriminate the origin and harvest year of 'Tempranillo' grape clones and with Partial Least Squares (PLS) regressions to predict its contents in soluble solids (SS), pH and titratable acidity (TA). Normalized spectra of grape homogenates and normalized plus 1st Derivative spectra of grape skins allowed an overall percentage of correct classifications of 99.6% and 96.7% in validation, according to origin, and 98.3% and 90.0% in validation, according to harvest year, respectively. The normalized spectra of grape homogenates allowed a calibration and validation determination coefficients (R2) of 0.92 and 0.90 for SS, 0.90 and 0.84 for pH, 0.88 and 0.84 for TA, respectively.The ATR-MIR combined with multivariate analysis showed to be an appropriate tool to assist the clonal selection process of 'Tempranillo'.
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