Abstract
Atrial natriuretic peptide (ANP), a 28 amino acid basic polypeptide, is known to induce histamine release from human and rat mast cells in vitro and cause a wheel formation in rat skin. However, cellular events associated with histamine release are not clearly understood. In this study, we have examined the calcium flux and cGMP formation associated with histamine release in the ANP-treated mast cells. ANP, in vitro, induced mast cell degranulation and histamine release in a dose-dependent manner. ANP also induced an enhanced calcium uptake into cells and increased the cellular level of cGMP in mast cells. A high level of calcium in the media caused an inhibition of ANP-dependent histamine release but enhanced the level of intracellular cGMP of mast cells. ANP inducing a dose-dependent increase in vascular permeability of rat skin was confirmed by the extravasation of the circulating Evans blue. The results indicate ANP induced the histamine release and an increase in vascular permeability through mast cell degranulation in cGMP-independent and calcium uptake-dependent manner.
Highlights
Mast cells are multifunctional effector cells of the immune
After the stimulation with 1 × 10−4 M of Atrial natriuretic peptide (ANP) for 5 min, rapid granule extrusion from the cell, disruption of the granular boundary, and swelling of cell shapes were observed indicating active degranulation of cell (Figure 1b). Such ANP-induced histamine release was a dosedependent: histamine release induced by 1 × 10−7, 1 × 10−6, 1 × 10−5, and 1 × 10−4 M of ANP amounted to 6.31 ± 0.20, 20.24 ± 3.92, 31.75 ± 3.37 and 57.63 ± 6.92% respectively (Figure 2a)
Calcium uptake induced by 1 × 10−7, 1 × 10−6, 1 × 10−5, and 1 × 10−4 M of ANP amounted to 107.40 ± 4.90, 220.60 ± 1.66, 233.70 ± 12.00 and 182.50 ± 2.91% respectively
Summary
Mast cells are multifunctional effector cells of the immune. Atrial natriuretic peptide (ANP) has been shown to release histamine from peritoneal mast cells and to increase dermal vascular permeability in rats (Opgenorth et al, 1990). ANP induced calcium uptake into the mast cells in a dose-dependent manner (Figure 2b).
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