Abstract
The aim of this study was to characterize and compare the effect of atrial natriuretic peptide (ANP) on ileal transport function in two common laboratory animals, the Hooded-Lister rat and the New Zealand White rabbit. ANP 1 μM produced a maximal increase in short circuit current ( I sc) that was Cl − dependent in both rat and rabbit. The maximal response in rat tissue was twice the magnitude of that seen in the rabbit. Furthermore, the rabbit I sc response was rapid and transient compared with that of the rat. In both rats and rabbits, the ANP response was dependent on extracellular Ca ++. Neural blockade had no effect on the rat ANP response but significantly inhibited the ANP response in rabbits. In the rat, the effect of ANP is mediated by seratonin (5-HT) acting through 5-HT 2 receptors. In contrast, no role for 5-HT could be seen in the rabbit ileal ANP response. In intact tissue in both rat and rabbit, ANP stimulated a significant rise in cGMP levels. ANP had no effect on cAMP levels in either species. The findings suggest a separate and distinct mechanism for ANP-mediated intestinal Cl − secretion in the rat ileum compared with the rabbit.
Published Version
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