Abstract
Using DPPH, ABTS and Folin-Ciocalteu procedures [1] the antioxidant capacity was characterized of fresh infusions of the raw AGCB distributed in Poland and originating from Central and South America. Next, UHPLC-TOF-MS/MS analyses of all infusions and MeOH extracts of raw AGCB were made using a Waters ACQUITY UPLC TabMode system interfaced with a Bruker microOTOF-Q mass spectrometer with an ESI source operating in negative mode at 150 °C and 4.5 kV with N2 nebulisation at 1.2 bar and a dry gas flow at 6 mL/min. An Agilent Poroshell 120EC-C18 column (2.1 × 150, 2.7 µm) was used operating at 0.6 mL/min flow rate with a mobile phase consisted of a linear gradient of 0.1% formic acid in water (A) and 0.1% formic acid in AcN (B), mixed by increasing eluate B in the range 5 – 90% from 0 – 20 min and followed by a split of column effluent flow 3:2 before the ESI ion source was used. MSMS spectra were analyzed with a Bruker Compass Data Analyzer v.4. The atractyligenin derivatives, i.e. 2-O-beta-D-glucopyranosyl-carboxyatractyligenin (m/z 525) (1), 2-O-(2'-O-isovaleryl-beta-D-glucopyranosyl)-carboxyatractyligenin (m/z 608) (2) and 3'-O-beta-D-glucopyranosyl-2'-O-isovaleryl-2-beta-(2-desoxy-carboxyatractyligenin)-beta-D-glucopyranoside (m/z 771) (3), were identified in the MeOH extracts of the raw but not milled AGCB from Colombia. Only compound 1 with a high inhibitory activity of adenine nucleotide translocase (ANT) [2] was confirmed by MSMS in the infusions of this AGCB sample. This indicates that some atractylosides in raw AGCB were hydrolyzed by hydrothermal treatment [3]. Compounds 1 and 3 with, respectively, significant and non-significant ANT inhibition were recently detected in the AGCB [2], but glucoside 2 was identified here for the first time.
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