Abstract
We have developed a stable and selective ATP biosensor for long-term in vitro tissue monitoring. The electrode was fabricated by entrapping glucose oxidase (GOx) and hexokinase (HEX) in a poly-phenol film on a Pt microelectrode. The biosensor was stable to a fixed concentration of glucose for over 20min and had a limit of detection of 9.9±3.2nM, with a sensitivity of 45.8±1.22pAμM−1. Most significantly of all, the response on the ATP biosensor did not alter in the presence of 1mM ascorbic acid, 5μM dopamine, 5μM serotonin, 5μM ADP and 5μM AMP. The ATP biosensor was also shown to have excellent stability over 7days, and showed only a 23.92±3.55% loss in sensitivity. The ATP biosensor was utilised for the in vitro detection of ATP from gastrointestinal tissue. The ATP biosensor response was stable for 5h during in vitro recordings from ileum tissue. ATP release was shown to be greater from the mucosal surface in the ileum compared to the colon.
Published Version
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