Abstract
We investigated the inhibitory effects of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) on ATP-dependent H + accumulation by membrane vesicles prepared from the turtle urinary bladder epithelium. NBD-Cl at 30 μM was found to completely inhibit the vanadate-insensitive component of H + transport, with half-maximal inhibition occurring at 4.2 to 5.4 μM. In contrast, the vanadate-inhibitable component was unaffected by 30 μM NBD-Cl. At high concentrations (300 μM), both components were fully inhibited. The results confirm the presence of two distinct H + transport processes in turtle bladder membranes and identify selective inhibitors, NBD-Cl and vanadate, for each process.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call