Abstract
ObjectivesIncreased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies.MethodsA pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR–RFLP methods.ResultsStatistically a significant difference of serum IgE levels were observed among cases and controls (P < 0.05). The observed significant difference of serum IgE levels were retained among subjects who also harboured variant genotypes of IL-4 and IL-13 genes (P < 0.05). Additionally, the above genetic variants significantly modified the risk of allergy when stratification was done based on various clinical characteristics.ConclusionOur study suggests that increased IgE levels and in association with variant forms of IL-4 and IL-13 genes are significantly associated with different types of allergies in study population.
Highlights
Allergic disorders are considered an epidemic in the developed world with rising incidence rate in the developing countries as well [1,2,3]
Patients and controls A total of 528 subjects presented with different allergic symptoms attending Allergy Clinic, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Department of Dermatology, SKIMS Medical College and Department of Dermatology, Sheri Maharaja Hari Sing (SMHS) Hospital Srinagar were included in this study
On the basis of age, the patients were grouped into two categories, less than 40 (< 40) and greater or equal to 40 (≥ 40) years of age
Summary
Allergic disorders are considered an epidemic in the developed world with rising incidence rate in the developing countries as well [1,2,3]. These disorders though multi-etiological but none has been established as a causative factor individually until now. The increased prevalence associated with large differences between urban and rural regions, hints at environmental factors. It results due to immune reaction to an antigen called an allergen, which is normally harmless [4, 5]. Allergen-specific IgE- antibodies cause most allergic reactions. Genome wide Association studies have identified strong linkage of a region on chromosome 5q31–33 with atopic disorders in different
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