Atomic absorption spectrometric determination of the iridium content in tumor cells exposed to an iridium metallodrug

Abstract

An electrothermal atomic absorption spectrometric method to quantify the iridium content of HT-29 colon carcinoma cells exposed to iridium metallodrugs was developed. Optimisation of the procedure involved the evaluation of pyrolysis and atomisation conditions (optimal values were 1400 °C for pyrolysis and 2400 °C for atomisation) and the addition of appropriate additives. The presence of cellular components as well as the addition of nitric acid and hydrochloric acid led to enhanced absorption signals and suggested the use of matrix matched calibration. The described method allows the measurement of iridium in cell suspensions in the low μg/L range (linear dynamic range: 10–450 μg/L) with a detection limit of 11.2 μg/L. The applicability of the method was tested by means of a novel iridium metallodrug. First results on the complex [IrCl 3(DMSO)(phen)] indicated a low cellular uptake (21.2 μM at incubation with 100 μM) of this iridium species in HT-29 cells compared to other metal containing antitumor drugs.