Asterias rubens in High Dilutions exhibiting Anti-Cancer Activity in Breast Cancer Cell Line: An in vitro Study

Abstract

Asterias rubens constitutes saponins that represents a class of steroid oligoglycoside namely asterosaponins that has broad biological functions inclusive of anti-cancerous activity. The most common malignancy as well as the second largest cause of mortality related to cancer in women is breast carcinoma. The prime objective of this research is to assess the anti-cancer activity of Asterias rubens in its higher dilutions on MCF-7 cells and to evaluate the process of angiogenesis. In vitro cytotoxicity was assessed by MTT and SRB assay. To evaluate the long-term survival capacity and proliferative ability of MCF-7 cell line, colony forming assay was performed. CAM assay was performed to evaluate the angiogenic potential of Asterias rubens. It was observed that Asterias rubens showed significant anti-cancer activity. In the in vivo study, chick embryo treated with Asterias rubens showed angiogenesis. Asterias rubens in higher dilutions exhibited anti-cancer activity, signifying its utility as supportive medicine in cancer treatment.