Abstract
This study was designed to assess the effects of dietary carotenoid supplementation on liver and kidney xenobiotic-metabolizing enzymes in the rainbow trout. Twelve rainbow trout (mean weight 266±10 g) were assigned to each of three replicate tanks for each of four dietary treatments; astaxanthin, canthaxanthin, negative control and positive control using β-naphthoflavone, at a target dietary inclusion of 100 mg kg −1 for each additive. Fish were fed for 3 weeks at a level of 1.2% body wt. day −1. Serum carotenoid levels were used as indicators of exposure and were not significantly different ( P>0.05) between carotenoid-fed trout. Livers and kidney were frozen separately in liquid N 2 by immersion and microsomal fractions from pooled samples ( n=3) assayed for xenobiotic-metabolizing enzyme (cytochrome P450 monoxygenase) activities including ethoxyresorufin O-deethylase; methoxyresorufin O-demethylase; pentoxyresorufin O-dealkylase; benzoxyresorufin O-dearylase; and the conjugating enzymes glucuronosyl transferase; and glutathione- s-transferase. Results revealed that carotenoid treatment did not significantly ( P>0.05) induce any enzyme system examined. Results are discussed in the context of metabolism of absorbed carotenoids.
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