Taurine supplementation in plant-based diets for juvenile rainbow trout (Oncorhynchus mykiss): Effects on growth performance, whole body composition, and histomorphological features

Abstract

Taurine supplementation in plant protein-based diets has been reported to improve growth performance, feed utilization, whole body composition, and gut integrity in carnivorous fish species, but data in rainbow trout are quite scarce. This study aimed to investigate the effects of plant protein-based diets supplemented with different levels of taurine on rainbow trout growth performance, whole body composition, and histomorphological traits of the intestine and main organs. In a 71-day trial, 340 juvenile rainbow trout were randomly distributed to 5 dietary treatments (4 replicate tanks/diet, 17 fish/tank): two control diets (a fishmeal-based diet [positive control; C+] and a soybean meal-soy protein concentrate-based diet [negative control; C-]), and three experimental diets in which the C-diet was supplemented with 2.5, 5 and 10 g/kg of taurine (T2.5, T5 and T10, respectively). At the end of the trial, growth parameters were assessed, and whole fish (12 pools of 3 fish/diet) and gut, liver and kidney samples (12 fish/diet) were collected for whole body composition and histomorphological analyses, respectively. Liver (8 fish/diet) was also sampled for taurine quantification. The fish fed the T10 diet showed higher weight gain, protein efficiency ratio, and specific growth rate – as well as decreased feed conversion ratio – when compared to the C+ group only (P < 0.05), thus suggesting a main effect of the taurine source on the response. The T2.5 rainbow trout showed lower moisture content and increased ether extract and ash contents than the other dietary treatments (P < 0.001). Higher crude protein content was also observed in fish fed the C- and T10 diets in comparison with the other groups (P < 0.001). Hepatic taurine levels were increased in the C+ group when compared to the C-, T2.5 and T5 diets (P < 0.05), with higher contents being also identified in the T10 group than the C- diet (P < 0.01). Differently, gut morphometric indices and histopathological features of the gut, liver, and kidneys were unaffected by dietary taurine supplementation (P > 0.05). In conclusion, dietary taurine supplementation in juvenile rainbow trout fed vegetable protein-based diet is capable of influencing the growth performance and the whole lipid content of the animals, without affecting the histomorphological traits of the intestine, the liver and the kidneys. In particular, the 2.5 and 10 g/kg supplementation levels seem to be capable of exerting the most pronounced effects in terms of composition of the fish body mass and growth parameters, respectively.