Abstract
An RNA linked DNA molecule has been isolated from T7 infected cells after pulse labeling with 3H-Tdr. This molecule binds almost exclusively to the R strand of T7 DNA. Recent results (1,2,3) have indicated that an RNA molecule may act as a primer to initiate DNA replication in bacteria and bacteriophage systems. Thus, at the initiation of DNA replication in bacteriophage T7 infected cells, one ought to be able to isolate a ribopolynucleotide covalently attached to a growing deoxypolynucleotide chain. In this communication, we report the isolation and partial characterization of such a molecule.
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