Abstract
Summary The associative effect of mixed cultures of Azotobacter and different rhizosphere fungi with Rhizobium japonicum on nodulation and symbiotic nitrogen fixation of soybean (Glycine max) was studied. It was found that mixed cultures of Azotobacter caused increase in root nodules, nodules fresh weight, dry weight of plant, and symbiotic nitrogen fixation. On the other hand, the mixed culture of rhizosphere fungi showed decrease in nodulation and symbiotic nitrogen fixation with the exception of Fusarium moniliforme, which revealed slight increase in nodulation and symbiotic nitrogen fixation. The organisms used in this study were also tested for growth-promoting substances. It was found that both Azotobacter and rhizosphere fungi synthesized in their culture medium gibberellin (GA3) and indole acetic acid (IAA).
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