Associations of two general ABCA1 polymorphisms and risk of coronary heart disease in north of Iran

Abstract

The aim of the present study was determined the frequency distribution of R1587K and I883M polymorphisms of the ABCA1 gene in the northern Iranian population and to assess their relationship with the Coronary Heart Diseases phenotype and lipid profile.A total of 199 blood samples were obtained from 85 cases with low HDL and 114 cases with high HDL. The biochemical test for the determination lipid profile was performed by commercial kit and then, PCR amplification was done by the specific primer of I883M and R1587K polymorphisms. The digestion of PCR products was done by two restriction enzymes, including Eco32I (EcoRV) and BauI (BssSI).The frequency of Allele K in low and high HDL populations was 0.62 and 0.60, respectively. There was no significant correlation between low levels of HDL and R1587K polymorphism. Being a carrier of allele K versus RR genotype increased the chance of low HDL OR = 0.80 times (95%, CI = 0.32–1.98). The frequency of Allele M was 0.73 and 0.77 in low and high HDL populations, respectively. There was not any significant association between low levels of HDL and I883M polymorphism. Being a carrier of allele M versus II genotype increased the chance of low HDL OR = 0.58 times (95%, CI = 0.15–2.23).Our results confirm the lack of significant associations between serum HDL levels and ABCA1 R1587K and I883M polymorphisms. Therefore, in this population cannot be expected that these polymorphisms as independent anticipation to identify subjects at risk for cardiovascular disease is suggested.