Associations Between Levels of Liver Enzymes and Circulating Antigens in Individuals Infected with Schistosomiasis Heamatobium

Samir Zaahkouk,Sawsan El-Shamy,Ibrahim Aly,Alaa Samn,Ibrahim Zahran

doi:10.21608/eajbsc.2020.134903

Abstract

Current estimates of the prevalence of schistosomiasis depend on the use of well-established, but imperfect, diagnostic tests where specimens are processed by routine parasitological methods and parasitological cure is judged by inspection of a cohort of children before and after Praziqantel (PQZ) treatment. In this study, in order to evaluate the different immunodiagnostic antigen detection assays, the selection of a proper antigen and its purification followed by propagation of its specific antibodies and purification were mandatory. Measurement of Liver function and kidney function were performed and its relation to S. heamatobium infection in different age and sex individuals were studied. Serum samples from an infected human with S. haematobium gave a strong reaction against S. haematobium SWAP antigen and no cross-reacting. with other parasites. The produced anti-S. haematobium SWAP IgG-pAb diluted 1/250 in PBS/T buffer gave strong reactivity to S. haematobium SWAP. The highest percentages of S. haematobium infected patients were in the age group 21-30 years (25%) and the least percentages of patients were in the age group of 51-55 years (2.9%). Levels of ALT , AST and ALP in the serum of humans among schistosomiasis group recorded a highly significant increase compared to the healthy control group. In conclusion strong association between biochemical parameters and serum circulating antigens among schistosomiasis infection.

Highlights

Schistosomiasis is one of the most widespread parasitic infections of man and is second to malaria in socioeconomic and public health importance in tropical and subtropical areas.Schistosomiasis affects 230 million of the world's poorest people through 77 countries in tropical Asia, Africa, South America and Caribbean
Antigen: One hundred viable worms of S. haematobium were incubated for 16 h at 37C° in Roswell Park Memorial Institute (RPMI) 1640 medium, 50 ml of supernatant were collected containing crude extract of Soluble worm antigen preparation (SWAP)
Serum samples from an infected human with S. haematobium gave a strong reaction against S. haematobium SWAP antigen with mean OD reading equal to 1.69 and no crossreactions were recorded with sera of patients infected with other parasites e.g., H.nana (8), Fasciola (8), Ascaris (8), and Hookworms (8). (Table 1 )

Summary

Introduction

Schistosomiasis is one of the most widespread parasitic infections of man and is second to malaria in socioeconomic and public health importance in tropical and subtropical areas.Schistosomiasis affects 230 million of the world's poorest people through 77 countries in tropical Asia, Africa, South America and Caribbean. Serum samples from an infected human with S. haematobium gave a strong reaction against S. haematobium SWAP antigen and no cross-reacting.

Results

Conclusion