Association study between cystathione-beta-synthase gene polymorphism and schizophrenia

Abstract

Objective To explore the relationship between cystathione-beta-synthase(CBS) gene T833C,G919A polymorphisms and schizophrenia. Methods The CBS gene polymorphism was detected by polymerase chain reaction and DNA sequencing technique in 93 patients with schizophrenia and 102 healthy people as normal control. Results ⑴ No CBS gene T833C ,G919A polymorphisms were found in all subjects,but mutation of G→A was detected at the 33th base in intron 8-9. Both heterozygote G/A and homozygote A/A were found in patient group,only heterozygote G/A was found in control group. ⑵ There was no significant difference of genotype distribution for mutation of G→A between patient group and control group(P>0.05). The frequency of A allele in patient group (8.07%)was higher than that in control group(3.43%) (χ2=3.92,P=0.048,OR=2.47,95%CI=1.01～6.05). ⑶ There was no significant difference of genotype distribution for mutation of G→A between clinic subgroups and control group(all P>0.05). The frequency of A allele in positive subgroup(8.70%) and later first-onset age subgroup(8.93%) were higher than that in control group(χ2=4.35,P=0.037,OR=2.68,95%CI=1.06～6.77; χ2=4.29,P=0.038,OR=2.76,95%CI=1.06～6.43). Conclusion The finding suggests that mutation of CBS gene T833C ,G919A is very low and it may be not correlated with schizophrenia. Mutation of G→A at the 33th base in intron 8-9 of CBS gene may be one of risk factors for schizophrenia with later first-onset age or positive symptom. Key words: Schizophrenia; Cystathione-beta-synthase; Genetics; Polymorphism