Association of vitamin D receptor and Toll like receptor variants with colon cancer related risk: A case control study in Egypt

Abstract

Variants of vitamin D receptor (VDR) and toll like receptors (TLR) have been investigated in relation to colon cancer (CC) susceptibility, however results were always inconsistent. MethodsWe examined the association of CC risk with four variants VDR rs2228570 (FokI), VDR rs11568820 (Cdx2) and Asp299Gly (rs4986790) and Asp399Ile (rs4986791) in TLR4. rs11568820 was assessed by allele specific-multiplex polymerase chain reaction, while the other variants were examined by restriction fragment length polymorphism. We evaluated whether any of the associations differed by tumor location or criteria. ResultsThe A allele of rs11568820 was associated with decreased risk with adjusted OR = 0.523 (0.344–0.796), p = 0.002, while G allele in Asp299Gly and T allele in Thr399Ile were associated with >3 fold increased risk of CC with OR = 3.430 (1.588–7.408), p = 0.002 and OR = 3.290 (1.628–6.648), p = 0.001 respectively. G allele of Asp299Gly was more strongly associated with distal tumors OR = 4.952 (2.171–11.29), p < 0.001 than proximal tumors OR = 2.401 (1.002–5.751), p = 0.043 with almost 2 fold rise in the OR. On the other hand rs11568820 A allele was more associated with proximal tumors OR = 0.574 (0.355–0.926), p = 0.022 than distal tumors OR = 0.400 (0.224–0.712), p = 0.001. ConclusionsWe conclude significant association of VDR rs11568820 (Cdx2) and TLR4 variants with CC, although results should be confirmed in additional studies.