Abstract
Toll-like receptors (TLRs) are essential in the host defense against microbial pathogens. Individual TLRs recognize distinct structural components of pathogens and evoke inflammatory responses. In the present study, the amplicon of 316 bp of TLR4 gene was amplified in the thermal cycler (Gene Amp 6700, Applied Biosystem) using the primers of TLR4 gene and the genetic polymorphism was detected by Single Strand Conformation Ploymorphism (SSCP). The SSCP analysis of 316 bp region of TLR4 gene revealed similar type of SSCP patterns in healthy, subclinical and clinical mastitis cases suggesting that Frieswal cattle is monomorphic for TLR4 gene.
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