Abstract
This study was carried out to investigate the relationship between common variants in two vitamin D pathway genes (VDR and CYP27B1) and vitamin D3 serum levels. In this study, serum vitamin D metabolite levels were measured in the blood samples of 200 patients with alopecia areata. Then, single nucleotide polymorphisms (SNPs) in VDR and CYP27B1 were analyzed using polymerase chain reaction (PCR)-sequencing. Sixty-three variations were observed in these genes (42 variations in CYP27B1 and 21 variations in VDR). A significant difference in Rs1544410 (odds ratio: 7, P < 0.0005) and rs4646536 (odds ratio: 4.043, P < 0.0005) variants was found between the patients and controls. The study showed the relationship between the two polymorphisms, Rs1544410 (odds ratio: 7, 95% CI, 1–8) and rs4646536 (odds ratio: 4.043, 95% CI, 3–14.038) on the genes VDR and CYP27B1, respectively, with increased risk of developing vitamin D3 insufficiency in the Iranian population. Therefore, SNPs in the VDR and CYP27B1 genes can be considered as prognostic biomarkers of the risk of developing vitamin D3 deficiency.
Highlights
Vitamin D is a group of biologically inactive, fat-soluble prohormones with known importance in maintaining body health
The study showed the relationship between the two polymorphisms, Rs1544410 and rs4646536 on the genes vitamin D receptor (VDR) and CYP27B1, respectively, with increased risk of developing vitamin D3 insufficiency in the Iranian population
As previous studies have shown a significant association between single nucleotide polymorphisms (SNPs) in the CYP27B1 and VDR genes and serum 25(OH)D3 levels, we investigated SNPs in these genes using the polymerase chain reaction (PCR)-sequencing method
Summary
Vitamin D is a group of biologically inactive, fat-soluble prohormones with known importance in maintaining body health. The accepted indicator of vitamin D3 sufficiency in humans is serum 25-hydroxyvitamin D3 (25(OH) D3) levels (normal levels defined as 25(OH)D3 serum levels from 30–100 ng/mL). The main causes of human vitamin D3 insufficiency (defined as serum 25(OH)D3 levels
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call