ASSOCIATION OF PON1, TNFα, AND TGFβ GENE POLYMORPHISMS WITH PROGNOSIS IN ORAL AND OROPHARYNGEAL SQUAMOUS CELL CARCINOMA

Abstract

The oral and oropharyngeal squamous cell carcinoma (OOSCC) accounts for 90% to 95% of tumors in the oral cavity. Single nucleotide polymorphism (SNP) in the coding region of PON1, TNFα, and TGFβ have been associated with the development of different cancers. Objective: Investigate the prognostic value of PON1 (rs854560 and rs662), TNFα (rs1800629 and rs361525), and TGFβ (rs1800469) SNPs in OOSCC. Study Design: One hundred sixty-three patients with OOSCC were genotyped along with 146 control patients for PON1 (rs854560 and rs662); TNFα (rs1800629 and rs361525); and TGFβ (rs1800469) SNPs by real-time polymerase chain reaction. Results: TNFα (rs1800629) GG genotype was significantly more frequent in intraoral lesions and clinical stages III and IV, while the polymorphic AA genotype was found predominantly in lip lesions and clinical stages I and II. Moreover, TGFβ (rs1800469) AG and AA genotypes were significantly more frequent in larger tumors (T3 e T4). The TNFα (rs1800629) AG genotype was associated with poor survival and patients carrying the PON1 (rs662) TT genotype also had a tendency for poor survival. CONCLUSIONS: The results suggest that the rs1800629 and rs1800469 variations could exert influence on the more aggressive behavior of OOSCC, and the genotypes AG of rs1800629 and TT of rs662 could be markers with prognostic value in OOSCC. The oral and oropharyngeal squamous cell carcinoma (OOSCC) accounts for 90% to 95% of tumors in the oral cavity. Single nucleotide polymorphism (SNP) in the coding region of PON1, TNFα, and TGFβ have been associated with the development of different cancers. Objective: Investigate the prognostic value of PON1 (rs854560 and rs662), TNFα (rs1800629 and rs361525), and TGFβ (rs1800469) SNPs in OOSCC. Study Design: One hundred sixty-three patients with OOSCC were genotyped along with 146 control patients for PON1 (rs854560 and rs662); TNFα (rs1800629 and rs361525); and TGFβ (rs1800469) SNPs by real-time polymerase chain reaction. Results: TNFα (rs1800629) GG genotype was significantly more frequent in intraoral lesions and clinical stages III and IV, while the polymorphic AA genotype was found predominantly in lip lesions and clinical stages I and II. Moreover, TGFβ (rs1800469) AG and AA genotypes were significantly more frequent in larger tumors (T3 e T4). The TNFα (rs1800629) AG genotype was associated with poor survival and patients carrying the PON1 (rs662) TT genotype also had a tendency for poor survival. CONCLUSIONS: The results suggest that the rs1800629 and rs1800469 variations could exert influence on the more aggressive behavior of OOSCC, and the genotypes AG of rs1800629 and TT of rs662 could be markers with prognostic value in OOSCC.