Association of particular systems with the release of neutral lipids inEchinostoma revolutum (Trematoda) adults

Abstract

Since free sterol excretory-secretory (E-S) products are involved in pheromonal communication in adultEchinostoma revolutum (Trematoda), attempts were made to associate specific systems with the release of lipids from this organism. A micropipet design was used to isolate neutral lipids from the excretory system versus those obtained from both the alimentary and the reproductive systems. Tegumentary lipids were obtained by rubbing the surface of worms with gauze. As determined by thin-layer chromatography, the major neutral lipid obtained from all systems was free sterol. Additional minor neutral lipid fractions were obtained from the excretory, alimentary, and reproductive systems. Histochemical oil red O studies showed neutral lipids only in the excretory system. Neutral lipids released from all of the above-mentioned systems may play a role in pheromonal communication in this species.