Association of mitochondrial function and sperm progressivity in slow- and fast-growing roosters

Abstract

The objectives of the current study were to investigate the activity of the electron transport chain (ETC) complexes, adenosine triphosphate (ATP), and reactive oxygen species (ROS) in the sperm mitochondria of 2 Iranian slow- and fast-growing chickens, namely native and Aryan strains. In this study, semen of 133 roosters was analyzed by computer-assisted sperm analysis (CASA). The roosters were categorized by sperm progressivity, as a low or high sperm motility phenotype. Mitochondrial complex I (NADH ubiquinone oxidoreductase), II (succinate dehydrogenase), III (ubiquinol cytochrome C reductase), IV (cytochrome C oxidase) activity, ATP, and ROS production in sperm were assayed. As a result, Aryan roosters with high progressive motility levels (HPL) had the greatest progressivity. Progressive motility was significant in in both strains (Aryan and native; P = 0.020) and motility levels (high and low; P = 0.007). The highest activity of complexes I was observed in Aryan with HPL roosters (P = 0.004). Native roosters with HPLs demonstrated higher complex activities I compared with Aryan and native roosters with low progressive motility levels (LPL) (P = 0.004). Significant differences were observed not only in the mitochondrial amounts of ATP of the strains (P = 0.000) but also between HPL and LPL (P = 0.003). The highest mitochondrial amounts of ATP was found in Aryan roosters with HPL (P = 0.021). Native roosters with LPL had the highest concentration of mitochondrial ROS (P = 0.033). The Aryan roosters with HPL, on the other hand, indicated less concentration of the mitochondrial ROS compared with Aryan having LPL and native roosters with HPL and LPL (P = 0.033). A significant difference was observed in the mitochondrial ROS between the strains (P = 0.004) and between HPL and LPL (P = 0.000). There was positive relationship between progressivity and each of mitochondrial complexes and ATP (r = 0.71, 0.62, 0.90 and 0.65 respectively). Based on our results, the sperm progressivity largely depends on the whole energy production originating in the mitochondrial compartment. Therefore, the deficiency in the function of mitochondria in the sperm and energy production could be responsible for low progressive motility.