Association of miR-34b/c gene polymorphisms and event-related potential P300 in major depressive disorder

Abstract

Objective To explore the relationship of miR-34b/c gene polymorphisms and event-related potential P300 in major depressive disorder. Methods The design of case-control research was used , and 302 major depressive patients and 327 normal controls who were in age and gender matched with patients were measured auditory event-related potential P300 on the day when two groups were collected.Polymerase chain reaction(PCR)and direct DNA sequencing technology were used to detect miR-34b/c gene polymorphisms. Results (1)In the single locus analysis, the rs4938723, rs2187473 and rs28757623 had no significant difference in allele frequency and genotype frequency between depressive patients and controls(P>0.05); Haplotype C-C-C in rs4938723-rs2187473-rs28757623 was statistically significant different in depressive patients and controls(χ2=3.96, P=0.046). The odds ratio (OR) was 1.322(95%CI=1.004-1.740). (2)Compared with normal controls, P300 of the patients with major depressive disorder had longer latency of N2(P<0.01), P3a(P<0.01)and P3b(P<0.05). (3)The P300 targets of major depressive disorder had statistical difference(P<0.05)in rs28757623 between the individuals with the G allele genotype and C/C genotype.The latency of N1 ((90.80±28.62)ms), P3a((281.79±37.89)ms), P3b((323.87±41.17)ms) were longer than C/C genotype ((77.40±20.96)ms, (253.00±34.36)ms, (297.30±23.70)ms). Conclusion Rs4938723-rs2187473-rs28757623 haplotype CCC in miR-34b/c gene might be risk factor for the onset of depression, miR-34b/c gene rs28757623 polymorphism is associated with the principal component of P300 latency in patients with Major depressive disorder which suggest that genetic factors may have a certain impact on cognitive function in the patients with major depressive disorder. Key words: miR-34b/c gene; Gene polymorphism; Major depressive disorder; Event-related potential P300