Abstract
In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examined in the control group. All patients underwent genetic testing by allele-specific polymerase chain reaction. In particular, 320A>C polymorphic locus of ММР20 gene; Val275Ala ММР20, and -8202A>G gene ММР9 were analyzed.We have found that 30 patients (71.4% of total) had bronchial asthma of mild severity, 9 children (21.4%) exhibited moderate degree, and 3 patients (7%) had severe-grade disease. Homozygous C/C variant of the polymorphic ММР20 gene, 320A>C heterozygous variant of the ММР20 Val275Ala polymorphism, and heterozygous locus of -8202A>G ММР9 gene were found to be most frequent among the children with asthma. Generally, we have observed that the frequencies of the studied alleles and genotypes did not significantly differ berween the asthma patients and children from the control group (p < 0.05). However, in patients with GGgenotype of -8202A>G ММР9 polymorphism combined with homozygosity for the C allele of ММР20 320A>C, a more severe disease was observed, being combined with polyvalent sensitization and high total IgE levels in blood serum.In conclusion, frequencies of alleles and genotypes among patients with asthma did not show any statistically significant differences from the group of healthy children. The patients homozygous for G allele of ММР9 -8202A>G polymorphism gene and for the C allele ММР20 gene (320A>C) seem to be predisposed for a more severe clinical course of the disease.
Highlights
Полиморфизмы генов MMP MMP gene polymorphism in bronchial asthma пластиночный фактор-4, субстанцию Р, амилоидный пептид Р), способствуя тем самым ремоделированию тканей [5]
In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children
Что у пациентов с генотипом GG по полиморфизму -8202A>G гена ММР9 и являющихся гомозиготами по С-аллелю полиморфизма 320А>С гена ММР20 наблюдается более тяжелое течение заболевания, сопряженное с поливалентной сенсибилизацией и повышенным уровнем общего IgE в сыворотке крови
Summary
Полиморфизмы генов MMP MMP gene polymorphism in bronchial asthma пластиночный фактор-4, субстанцию Р, амилоидный пептид Р), способствуя тем самым ремоделированию тканей [5]. Поэтому изучение ассоциации различных полиморфных вариантов генов ММП с клиническими проявлениями у детей, страдающих БА, представляет большой практический интерес. Проведенное генетическое исследование позволило изучить ассоциацию различных полиморфных вариантов генов ММП с риском развития БА у детей и сопоставить различные генотипы с клиническими проявлениями заболевания
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
