Association of Mannose-Binding Lectin 2 (mbl2) gene heterogeneity and its serum concentration with osteoporosis in postmenopausal women

Emina Kiseljaković,Bakir Mehić,Amina Valjevac,Elma Kučukalić-Selimović,Radivoj Jadrić,Slavka Ibrulj,Mirela Mačkić-Đurović,Sabaheta Hasić

doi:10.17305/bjbms.2014.2292

Abstract

The aim of the study was to detect prevalence of MBL2 exon 1 (codons 52, 54 and 57) genetic polymorphism in postmenopausal women in Bosnia and Herzegovina and its possible role as genetic risk factor for susceptibility to occurrence of osteoporosis in this study group. Also, we investigated association between MBL serum concentrations and osteoporosis in postmenopausal women. Genetic codons' variations were determined by PCR-RFLP and MBL in serum was measured by ELISA method in 75 postmenopausal women (37 with osteoporosis and 38 apparently healthy, non-osteoporotic women serving as a control). Serum MBL levels were not significantly different between osteoporosis and control group (492 (37-565.1) and 522.6 (477-559.4) ng/mL respectively, p=0.206). Genotype frequencies were not significantly different (p=0.997) between the studied groups of postmenopausal women. Genotype frequencies A/A, A/0 and 0/0 in osteoporosis group were 0.576; 0.405; 0.018 and in control group 0.562; 0.412; 0.026, respectively. Frequencies of A and 0 allele were 0.78 and 0.22 in osteoporosis and 0.77 and 0.23 in control group. The results do not suggest association of functional polymorphism of MBL2 gene and MBL serum concentration with osteoporosis in postmenopausal females.

Highlights

Mannose-binding lectin (MBL) is in focus of attention because of its role as a recognition molecule in complement system
The osteoporosis shows an inflammatory character [ ] and MBL is considered as modifier of inflammatory responses, we investigated association of MBL gene heterogeneity and MBL serum concentration with osteoporosis
It was shown that serum level of MBL is influenced by presence of genetic polymorphism at the protein coding region consist of four exons [ ]

Summary

Introduction

Mannose-binding lectin (MBL) is in focus of attention because of its role as a recognition molecule in complement system. It is Ca +-dependent collagenous lectin, synthesized in liver with main role to mediate innate immune defence against microorganisms. It is clear that MBL gene harbor complex genetic system associated with infectious conditions and it may be a diseases modifier in patients with certain diseases. The osteoporosis shows an inflammatory character [ ] and MBL is considered as modifier of inflammatory responses, we investigated association of MBL gene heterogeneity and MBL serum concentration with osteoporosis. It was shown that serum level of MBL is influenced by presence of genetic polymorphism at the protein coding region consist of four exons [ ].

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