Association of LTC4S gene rs730012 single nucleotide polymorphism with childhood asthma

Abstract

Objective To investigate the association between the single nucleotide polymorphism(SNP) of leukotriene C4 synthase(LTC4S) rs730012 in the childhood asthma. Methods Sequence specific primers-polymerase chain reaction was used to assess the genetic polymorphism of LTC4S rs730012 in 105 asthma children with different order of severity and 128 non-asthma children in our hospital in the northeast of China to analyse the association between the SNP of LTC4S rs730012 and susceptibility, clinical phenotype in asthma children. Results (1)In case group, genotype frequencies of A/C, A/A and C/C were 71.4%、25.7%、2.9%, allele frequencies for A, C were 84.3%, 15.7%.In control group, the genotype frequencies of A/A, A/C, C/C, were 70.3%, 28.9%, 0.8%, allele frequencies for A, C were 84.8%, 15.2%.No significant difference was found in AA genotype and C allele frequencies between case and control group(χ2=0.035、0.020, P both>0.05). (2)C/C genotype or C allele frequencies in moderate-severe asthma group were significantly higher than the mild asthma group(χ2=5.859、5.641, P both<0.05); (3) SaO2 of A/A group was significantly higher than A/C and C/C group(t=2.976, Pboth<0.05), and FeNO and obstructive ventilatory disorder incidence rate in A/C, C/C group were higher than A/A group, the differences were statistically significant(t=2.946、χ2=5.564, P both<0.05). Conclusion The SNP of LTC4S rs730012 is associated with the order of severity, SaO2, FeNO, pulmonary function in asthma children of northeast China.However, the rs730012 is not associated with the susceptibility for asthma. Key words: LTC4S; Gene polymorphism; Childhood asthma; Severity