Association of LOX-1 and CX3CR1 with coronary artery stenosis disease and its outcomes

Abstract

Objective To explore the association of lectin-like oxidized low-density lipoprotein (ox-LDL) receptor -1 (LOX-1), CX3C chemokine receptor 1 (CX3CR1) with coronary artery stenosis disease and its outcomes. Methods A case-control study was conducted.A total of 176 cases of coronary artery stenosis which were confirmed coronary artery stenosis ≥ 50% by coronary angiography(CAG) were served as case group from department of cardiology of TEDA International Cardiovascular Hospital of Tianjin from May 2011 to April 2013.A total of 129 patients without coronary artery lesion by CAG from this hospital in the same period were served as control group, which has no history of heart disease, liver and kidney dysfuction, brain disease, hematological disease, other disorders that could bring out atherosclerosis and thrombosis.General information and laboratory parameters, LOX-1, CX3CR1, uric acid (UA) and creatinine (CREA) were measured in 2 groups.These parameters of each group were compared,the levels of LOX-1 and CX3CR1 in one-vessel stenosis were compared than that in multi-vessels stenosis in case group, the correlations between LOX-1,CX3CR1 and Gensini score and other variables were analyzed.Comparison of the levels of LOX-1 and CX3CR1 between major adverse cardiovascular events (MACEs) group and non-major adverse cardiovascular event (MACE) group was made during follow up 1.5 years.MACEs in patients with different levels of LOX-1 and CX3CR1 were compared during 1.5-year follow up.All of the data were analyzed by SPSS 16.0 software.The independent-samples T test, Mann-Whitney U test, Chi-square test, Spearman correlation, Binary Logistic Regression and Kaplan-Meier probability were adopted for data analysis. Results Comparison between case group and control group, LOX-1:3.72(1.44，8.15) μg/L vs 0.75(0.50，1.19) μg/L，z=11.072，P 0.05).LOX-1 was negatively related to left ventricular ejection fraction(LVEF) (R=-0.272，P<0.01), but positively related to left ventricular end-diastolic diameter (LVDD)(R=0.190，P<0.05), positively related to UA (R=0.121，P<0.05).Comparison between MACE group and non-MACE group, LOX-1：7.38（4.97,11.88）μg/L vs 3.52(1.45,7.75) μg/L, z=2.762，P<0.01；CX3CR1：（4.02±2.90） μg/L vs （2.67±1.48) μg/L，t=3.086，P<0.01.LOX-1 and TG were independent risk effects of coronary artery stenosis disease.MACEs were increased in patients with high levels of LOX-1 after PCI during following up 1.5 years (comparison between high-LOX-1 group and low-LOX-1 group, the probability of non-MACE was 87.1% (115/132) vs 97.7%(43/44), Log-ranK test, χ2=6.957, P<0.01). Conclusions LOX-1 and CX3CR1 may be involved in the process of coronary artery stenosis, and a high level of LOX-1 may be associated with left ventricular systolic dysfunction in patients with coronary artery stenosis.Elevated LOX-1 level are closely related to afterwards MACE incidence after PCI in patients with coronary artery stenosis.（Chin J Lab Med,2014,37:66-71） Key words: Coronary stenosis; Scavenger receptors, class E; Receptors, chemokine; Prognosis