Abstract
Introduction:Leptin is a hormone secreted from adipocytes that regulates metabolism and energy homeostasis through the leptin receptor (LEPR). The aim of this study was to investigate the association of leptin receptor gene Q223R gene polymorphism, and plasma leptin level among obese breast cancer females. Materials and Methods:The study enrolled 160 breast cancer patients and 160 healthy control females. LEPR Q223R polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum leptin was determined using enzyme-linked immunosorbent assay human leptin kit. Immunohistochemical tests from paraffin blocks were carried out for estrogen and progesterone staging using the precise antibodies. Results:An association was found between LEPR gene Q223R gene polymorphism among obese breast cancer females. Statistical difference was found between GG (60.6%) Arg/Arg genotype (OR=2.986; 95%CI=1.540 to 5.789; p= 0.001) compared to AA (33.1%) Gln/Gln genotype. GG Q223R LEPR polymorphism showed statistically significant difference among obese breast cancer patients (BMI more than 25) compared to control (P < 0.0001). GG genotype of Q223R LEPR polymorphism showed statistically significant increased leptin level (p-value =0.0001) among obese patients (mean± SD; 23.39±4.32) compared to control (17.83±5.67). Conclusions:Q223R LEPR polymorphism GG genotype was associated with increased leptin profile among obese breast cancer females.
Highlights
Leptin is a hormone secreted from adipocytes that regulates metabolism and energy homeostasis through the leptin receptor (LEPR)
The aim of this study was to investigate the association of leptin receptor gene Q223R gene polymorphism, and plasma leptin level among obese breast cancer females
An association was found between LEPR gene Q223R gene polymorphism among obese breast cancer females
Summary
Leptin is a hormone secreted from adipocytes that regulates metabolism and energy homeostasis through the leptin receptor (LEPR). GG Q223R LEPR polymorphism showed statistically significant difference among obese breast cancer patients (BMI more than 25) compared to control (P < 0.0001). GG genotype of Q223R LEPR polymorphism showed statistically significant increased leptin level (p-value =0.0001) among obese patients (mean± SD; 23.39±4.32) compared to control (17.83±5.67). The four mechanisms that link breast cancer with obesity include: leptin and leptin receptor expression, adipose chronic inflammation, sex hormone alternation, and insulin and insulin like growth factor 1 (IGF-1) signaling (Atoum et al, 2017; Atoum et al, 2020). Elevated blood leptin concentrations or increased leptin receptors expression are associated with poor prognosis, lymph node involvement and cancer metastasis (Tsung-Chieh et al, 2021)
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