Abstract
Objective:The aim of study was to investigate the association of IL 1B gene polymorphism with involvement of H. pylori and other gastric diseases.Methods:Blood samples of dyspeptic patients were collected from endoscopy department of Allied Hospital Faisalabad from January 2017 to January 2019 and were qualitatively assayed for serological detection of CagA H. pylori antibodies. PCR followed by direct sequencing was performed for proinflammatory IL-1B gene polymorphism detection. Sequence analysis was performed in software SnapGene viewer for haplotypes.Results:Demographic characteristics of seropositive patients showed maximum 25% gastritis in age groups of 20-40 years and 41-60 years, predominantly (41.7%) in females. While in seronegative patient’s gastritis (33.3%) was found in age group of 20-40 years mainly in males (41.7%). Among studied groups, higher expression of IL-1B-511 genotype (33.3%) polymorphism was found in healthy individuals as compared to H. pylori seropositive (25%) and seronegative (8.3%). While IL-1B-31 genotype showed maximum 33.3% polymorphism rate in seropositive gastric diseased group. Moreover, haplotypes frequencies IL-1B-511CC and IL-1B-31TT were predominantly (20%) found in seropositive gastric diseased group.Conclusions:In H. pylori seropositive patients, gastric disease was commonly found, however, gastric disease was not only associated with H. pylori as seronegative patients were also carrying gastric complications. Interleukin IL-1B polymorphism was partially associated with H. pylori infection in studied dyspeptic population.
Highlights
Helicobacter pylori (H. pylori) is a Gram-negative rod shape bacterium that colonizes stomach of about 50% of world’s population.[1]
Demographic characteristic of gastric diseased patients showed that 50% of population were found in second age group, 28.6% in first while only 21.4% were found in third age group
Maximum 25% polymorphism was found at IL-1B-511CT in H. pylori seropositive gastric diseased cases, while for IL-1B-31TC genotype
Summary
Helicobacter pylori (H. pylori) is a Gram-negative rod shape bacterium that colonizes stomach of about 50% of world’s population.[1] It can colonize the gastric mucosal environment for years if left untreated and increase the risk of gastric disease development.[2] Frequent physiological changes by this bacterium are gastritis, peptic ulcer (PU) and less commonly gastric cancer.[3] H. pylori has been classified as class I carcinogen by the World Health Organization.[4] H. pylori is considered as a major cause of peptic ulcer and mucosa associated lymphoid tissue lymphoma or gastric cancer.[5] gastritis to gastric cancer development
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
