Abstract
PURPOSE: To study the distribution of human leukocyte antigen HLA-A/B antigens and HLA-DR/-DQ/-DP alleles and to investigate the immunogenetic background of Korean patients with Vogt-Koyanagi-Harada (VKH) syndrome and clinical course with different types of HLA. METHODS: Human leukocyte antigen typings were performed in 18 Korean patients with VKH syndrome and in 128 healthy control subjects. HLA-A/B loci serologic typing was performed according to the standard microlymphocytotoxicity technique. DNA was extracted through the salting out method, and HLA-DR phenotyping and HLA DR4, HLA-DQ, and HLA-DP subtyping were performed with the polymerase chain reaction- sequence specific oligonucleotide probe (PCR-SSOP) method. RESULTS: Among HLA-A/B antigens typed by the standard microlymphocytotoxicity method, the frequencies of HLA-A31 (RR = 6.1, P < 1 × 10 −2) and HLA-B55 (RR = 15.8, P < .05) were significantly increased in the patient group compared with the control group. Among HLA-DR/ -DQ/-DP alleles subtyped by DNA methods, the frequencies of HLA-DRB1∗04 (RR = 45.1, P < 1 × 10 −7) and HLA-DRB1∗07 (RR = 3.2, P < .05) were significantly increased. However, significant decreases in HLA-DRB1∗08 (RR = .1, P < .05), HLA-DRB1∗13 (RR = .1, P < .05), and HLA-DRB1∗14 (RR = .1, P < .05) frequencies were observed. The result of HLA-DR, HLA-DQ, and HLA-DP subtying showed the significant increase in DRB1∗0405 (RR = 45.1, P < 1 × 10 −7), DQA1∗0302 (RR = 12.0, P < 1 × 10 −4), DQB1∗0303 (RR = 5.0, P < 1 × 10 −2), DQB1∗0401 (RR = 18.9, P < 1 × 10 −6), and DPB1∗0501 (RR = 3.8, P < .05). However, significant decreases in DQA1∗0101 (RR = .1, P < .05), DQA1∗0102 (RR = .1, P < 1 × 10 −2), DQA1∗0103 (RR = .1, P < .05), DQA1∗0501 (RR = .1, P < 1 × 10 −2), DQB1∗0301 (RR = .1, P < .05), DQB1∗0601 (RR = .1, P < .05), DPB1∗0201 (RR = .3, P < .05), and DPB1∗0401 (RR = .1, P < .05) frequencies were also observed. In patients with DRB1∗0405 itself or HLA-DRB1∗0405-DQA1∗ 0302-DQB1∗0401 haplotype, a reduction in visual acuity and ocular complications was common. CONCLUSIONS: These results suggest that HLA-DRB1∗0405 itself or HLA-DRB1∗0405-DQA1∗ 0302-DQB1∗0401 haplotype is greatly increased and may play the most important role in the development and the clinical course of VKH syndrome in Korean patients.
Published Version
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