Association of CYP19A1 rs743572 Polymorphism with Breast Cancer Risk Factor in Iraqi Women- Case Control Study

Abdul-Hussein Al Faisal ,Iman Al-Bedairy

doi:10.7176/jmpb/66-05

Abstract

Breast cancer (BC) is the most common cancer and the second cause of mortality in women all around the world. It is caused by several factors including genetic determinants, so that both genetic susceptibility factors and environmental factors are involved in the etiology. Significance of genes functioning in steroid hormone synthesis and metabolism are well established in breast cancer susceptibility. Polymorphisms of genes encoding enzymes involved in estrogen biosynthesis pathway and in the metabolic activation of pro-carcinogens to genotoxic intermediates, such as cytochrome P450C17α (CYP17) was closely examined in this women-based case control study of Iraqi women aged range (27–91). The associations between selected single nucleotide polymorphisms (SNPs) in the CYP17 gene and breast cancer for 105 women with breast cancer and 120disease- free controls were evaluated. Restriction fragment length polymorphism (RFLP) technique was used with MspA1 digested enzyme for the PCR products. There was no significant C→T (rs743572) CYP17 associated with breast cancer in the Iraqi women studied. Keywords: breast cancer, CYP17, P450, SNPs. DOI: 10.7176/JMPB/66-05 Publication date: June 30th 2020

Highlights

Breast cancer (BC), is a disorder of cell growth
Much interest has long been focused on cytochrome P450c17α (CYP17) and glutathione S-transferase 1 (GSTP1) genes, encoding enzymes involved in estrogen biosynthesis and metabolism [14] which encodes steroid (17-alphahydroxylase) known as a steroid 17-alpha-monooxygenase
The final screening of the samples investigated the frequency of T→C substitution at -34bp from the initiation site of translation of gene CYP17 polymorphic (A2 allele of gene CYP17) in Iraqi women patients presenting with breast cancer ER+ and in matched controls, there was statistically significant by P-value 0.0472 with O.R. (C.I.) 0.772 (P≤0.05) for Heterozygous genotyping (A1A2) TC in control samples

Summary

Introduction

Breast cancer (BC), is a disorder of cell growth. BC is a dynamic population of abnormal somatic cells evolving through natural selection [1] (Fortunato at el. 2017). The risk of breast cancer is related to genetic, environmental, and lifestyle factors that influence the level of exposure to estrogens and other sex hormones [3,4,5] (Feigelson et al, 1996, Lichtenstein et al, 2000, Bhatia, 2015). Much interest has long been focused on cytochrome P450c17α (CYP17) and glutathione S-transferase 1 (GSTP1) genes, encoding enzymes involved in estrogen biosynthesis and metabolism [14] which encodes steroid (17-alphahydroxylase) known as a steroid 17-alpha-monooxygenase. It performs both 17-alpha-hydroxylase and 17, 20-lyase activity.

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Discussion

Conclusion