Association of circulating BDNF levels with BDNF rs6265 polymorphism in schizophrenia

Abstract

Schizophrenia is a severe neuropsychiatric disorder affecting 1% of the world population. Disturbances in neuronal development and synaptic connections are important factors in the pathogenesis of schizophrenia. Brain derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays a critical role in the development of neurons. Among several polymorphisms reported in BDNF, the rs6265 polymorphism is known to be associated with many neuropsychiatric diseases. This study was aimed to determine the effect of BDNF rs6265 functional polymorphism on serum BDNF concentration in patients with schizophrenia. In total, 50 schizophrenia patients and 50 controls were recruited after obtaining written informed consent. Serum BDNF levels were estimated using the ELISA method and BDNF rs6265 polymorphism was genotyped using T-ARMS PCR. Serum BDNF levels were decreased significantly in schizophrenia patients when compared to the healthy controls (p < 0.0001). Further, the rs6265 polymorphism was also not associated with the schizophrenia (p = 0.41). Intragroup analysis between different genotypes revealed no association between the serum BDNF levels and rs6265 polymorphism. Our results suggest that the functional polymorphism rs6265 is not associated with serum BDNF levels, which is in line with previous findings, which indicates that serum BDNF levels depend more on diagnostic effect than genetic effect. Replication studies on a larger study population are needed.